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d7 embryo marathon  (TaKaRa)


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    Structured Review

    TaKaRa d7 embryo marathon
    D7 Embryo Marathon, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryo+cdna/Mouse+7-day+Embryo+Marathon+-Ready+cDNA/bio_rxiv__64898__2025__12__18__695332-207-14-24
    Average 93 stars, based on 32 article reviews
    d7 embryo marathon - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Molecular antigen array
    Article Snippet: The fragment of mVEGFR-2 cloned into pCEP-SP-EK-Fc* vector encoded the following amino acid sequence (SEQ ID NO:345): PFIAS VSDQHGIVYI TENKNKTVVI PCRGSISNLN VSLCARYPEK RFVPDGNRIS WDSEIGFFLP SYMISYAGMV FCEAKINDET YQSIMYIVVV VGYRIYDVIL SPPHEIELSA GEKLVLNCTA RTELNVGLDF TWHSPPSKSH HKKIVNRDVK PFPGTVAKMF LSTLTIESVT KSDQGEYTCV ASSGRMIKRN RTFVRVHTKP Expression of Recombinant mVEGFR-2(2-3) in Eukaryotic Cells Recombinant mVEGFR-2(2-3) was expressed in EBNA 293 cells using the pCEP-SP-EK-Fc* vector. .. The mVEGFR-2(2-3) was amplified by PCR with the primer pair BamH1-FLK1-F and Nhe1-FLK1-B from a mouse 7-day embryo cDNA (Marathon-Ready cDNA, Clontech). .. The mVEGFR-2(2-3) was amplified by PCR with the primer pair BamH1-FLK1-F and Nhe1-FLK1-B from a mouse 7-day embryo cDNA (Marathon-Ready cDNA, Clontech).

    Article Title: Molecular antigen array
    Article Snippet: The fragment of mVEGFR-2 cloned into pCEP-SP-EK-Fc* vector encoded the following amino acid sequence (SEQ ID NO:345): PFIAS VSDQHGIVYI TENKNKTVVI PCRGSISNLN VSLCARYPEK RFVPDGNRIS WDSEIGFTLP SYMISYAGMV FCEAKINDET YQSIMYIVVV VGYRIYDVIL SPPHEIELSA GEKLVLNCTA RTELNVGLDF TWHSPPSKSH HKKIVNRDVK PFPGTVAKMF LSTLTIESVT KSDQGEYTCV ASSGRMIKRN RTFVRVHTKP Expression of Recombinant mVEGFR-2(2–3) in Eukaryotic Cells Recombinant mVEGFR-2(2–3) was expressed in EBNA 293 cells using the pCEP-SP-EK-Fc* vector. .. The mVEGFR-2(2–3) was amplified by PCR with the primer pair BamHI-FLK1-F and NheI-FLK1-B from a mouse 7-day embryo cDNA (Marathon-Ready cDNA, Clontech). .. The mVEGFR-2(2–3) was amplified by PCR with the primer pair BamHI-FLK1-F and NheI-FLK1-B from a mouse 7-day embryo cDNA (Marathon-Ready cDNA, Clontech).

    Article Title: Vascular endothelial cell growth factor antagonists and uses thereof
    Article Snippet: .. A murine flt-IgG (referred to as mflt(1-3)-IgG) was constructed by PCR amplification of mouse 17-day embryo cDNA (Clontech, Palo Alto, Calif.) using primers described in Ferrara et al., supra. ..

    Article Title: Vascular endothelial cell growth factor antagonists and uses thereof
    Article Snippet: .. A murine flt-IgG (referred to as mflt(1-3)-IgG) was constructed by PCR amplification of mouse 17-day embryo cDNA (Clontech, Palo Alto, Calif.) using primers described in Ferrara et al., supra. ..

    Polymerase Chain Reaction:

    Article Title: Molecular antigen array
    Article Snippet: The fragment of mVEGFR-2 cloned into pCEP-SP-EK-Fc* vector encoded the following amino acid sequence (SEQ ID NO:345): PFIAS VSDQHGIVYI TENKNKTVVI PCRGSISNLN VSLCARYPEK RFVPDGNRIS WDSEIGFFLP SYMISYAGMV FCEAKINDET YQSIMYIVVV VGYRIYDVIL SPPHEIELSA GEKLVLNCTA RTELNVGLDF TWHSPPSKSH HKKIVNRDVK PFPGTVAKMF LSTLTIESVT KSDQGEYTCV ASSGRMIKRN RTFVRVHTKP Expression of Recombinant mVEGFR-2(2-3) in Eukaryotic Cells Recombinant mVEGFR-2(2-3) was expressed in EBNA 293 cells using the pCEP-SP-EK-Fc* vector. .. The mVEGFR-2(2-3) was amplified by PCR with the primer pair BamH1-FLK1-F and Nhe1-FLK1-B from a mouse 7-day embryo cDNA (Marathon-Ready cDNA, Clontech). .. The mVEGFR-2(2-3) was amplified by PCR with the primer pair BamH1-FLK1-F and Nhe1-FLK1-B from a mouse 7-day embryo cDNA (Marathon-Ready cDNA, Clontech).

    Article Title: Molecular antigen array
    Article Snippet: The fragment of mVEGFR-2 cloned into pCEP-SP-EK-Fc* vector encoded the following amino acid sequence (SEQ ID NO:345): PFIAS VSDQHGIVYI TENKNKTVVI PCRGSISNLN VSLCARYPEK RFVPDGNRIS WDSEIGFTLP SYMISYAGMV FCEAKINDET YQSIMYIVVV VGYRIYDVIL SPPHEIELSA GEKLVLNCTA RTELNVGLDF TWHSPPSKSH HKKIVNRDVK PFPGTVAKMF LSTLTIESVT KSDQGEYTCV ASSGRMIKRN RTFVRVHTKP Expression of Recombinant mVEGFR-2(2–3) in Eukaryotic Cells Recombinant mVEGFR-2(2–3) was expressed in EBNA 293 cells using the pCEP-SP-EK-Fc* vector. .. The mVEGFR-2(2–3) was amplified by PCR with the primer pair BamHI-FLK1-F and NheI-FLK1-B from a mouse 7-day embryo cDNA (Marathon-Ready cDNA, Clontech). .. The mVEGFR-2(2–3) was amplified by PCR with the primer pair BamHI-FLK1-F and NheI-FLK1-B from a mouse 7-day embryo cDNA (Marathon-Ready cDNA, Clontech).

    Article Title: Polypeptide
    Article Snippet: .. PCR was performed to obtain a gene of which the expression was increased in a diabetes model as shown in Referential Example described below, by using mouse 11-day embryo cDNA and mouse 17-day embryo cDNA (Marathon-Ready cDNA; Clontech) as a template, DNA consisting of the base sequence of SEQ ID NO: 16 as a 5′ primer, DNA consisting of the base sequence of SEQ ID NO: 17 as a 3′ primer, and LAtaqDNA polymerase (Takara Shuzo). ..

    Article Title: Polypeptide
    Article Snippet: .. PCR was performed to obtain a gene of which the expression was increased in a diabetes model as shown in Referential Example described below, by using mouse 11-day embryo cDNA and mouse 17-day embryo cDNA (Marathon-Ready cDNA; Clontech) as a template, DNA consisting of the base sequence of SEQ ID NO: 16 as a 5′ primer, DNA consisting of the base sequence of SEQ ID NO: 17 as a 3′ primer, and LAtaqDNA polymerase (Takara Shuzo). ..

    Article Title: Vascular endothelial cell growth factor antagonists and uses thereof
    Article Snippet: .. A murine flt-IgG (referred to as mflt(1-3)-IgG) was constructed by PCR amplification of mouse 17-day embryo cDNA (Clontech, Palo Alto, Calif.) using primers described in Ferrara et al., supra. ..

    Article Title: Vascular endothelial cell growth factor antagonists and uses thereof
    Article Snippet: .. A murine flt-IgG (referred to as mflt(1-3)-IgG) was constructed by PCR amplification of mouse 17-day embryo cDNA (Clontech, Palo Alto, Calif.) using primers described in Ferrara et al., supra. ..

    Expressing:

    Article Title: Polypeptide
    Article Snippet: .. PCR was performed to obtain a gene of which the expression was increased in a diabetes model as shown in Referential Example described below, by using mouse 11-day embryo cDNA and mouse 17-day embryo cDNA (Marathon-Ready cDNA; Clontech) as a template, DNA consisting of the base sequence of SEQ ID NO: 16 as a 5′ primer, DNA consisting of the base sequence of SEQ ID NO: 17 as a 3′ primer, and LAtaqDNA polymerase (Takara Shuzo). ..

    Article Title: Polypeptide
    Article Snippet: .. PCR was performed to obtain a gene of which the expression was increased in a diabetes model as shown in Referential Example described below, by using mouse 11-day embryo cDNA and mouse 17-day embryo cDNA (Marathon-Ready cDNA; Clontech) as a template, DNA consisting of the base sequence of SEQ ID NO: 16 as a 5′ primer, DNA consisting of the base sequence of SEQ ID NO: 17 as a 3′ primer, and LAtaqDNA polymerase (Takara Shuzo). ..

    Sequencing:

    Article Title: Polypeptide
    Article Snippet: .. PCR was performed to obtain a gene of which the expression was increased in a diabetes model as shown in Referential Example described below, by using mouse 11-day embryo cDNA and mouse 17-day embryo cDNA (Marathon-Ready cDNA; Clontech) as a template, DNA consisting of the base sequence of SEQ ID NO: 16 as a 5′ primer, DNA consisting of the base sequence of SEQ ID NO: 17 as a 3′ primer, and LAtaqDNA polymerase (Takara Shuzo). ..

    Article Title: Polypeptide
    Article Snippet: .. PCR was performed to obtain a gene of which the expression was increased in a diabetes model as shown in Referential Example described below, by using mouse 11-day embryo cDNA and mouse 17-day embryo cDNA (Marathon-Ready cDNA; Clontech) as a template, DNA consisting of the base sequence of SEQ ID NO: 16 as a 5′ primer, DNA consisting of the base sequence of SEQ ID NO: 17 as a 3′ primer, and LAtaqDNA polymerase (Takara Shuzo). ..

    Construct:

    Article Title: Vascular endothelial cell growth factor antagonists and uses thereof
    Article Snippet: .. A murine flt-IgG (referred to as mflt(1-3)-IgG) was constructed by PCR amplification of mouse 17-day embryo cDNA (Clontech, Palo Alto, Calif.) using primers described in Ferrara et al., supra. ..

    Article Title: Vascular endothelial cell growth factor antagonists and uses thereof
    Article Snippet: .. A murine flt-IgG (referred to as mflt(1-3)-IgG) was constructed by PCR amplification of mouse 17-day embryo cDNA (Clontech, Palo Alto, Calif.) using primers described in Ferrara et al., supra. ..



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    Image Search Results


    Figure 1. Isolation of novel binding partners of HMX3 with yeast two-hybrid screening. Top: Schematic representation of bait and prey constructs used in screening. Full-length mouse HMX3 fused to the Gal4 DNA- binding domain (DBD) was used as bait. A library of stage E11 mouse cDNAs fused to the Gal4 activating domain (AD) was used as prey. 7.2 × 108 solid plate matings were performed, yielding 3200 positive colonies. Positive prey inserts were segregated and sequenced and reads were aligned to the mouse genome, identifying 851 unique genes. After removing non-coding genes and those for which only untranslated region (UTR) or intronic sequences were recovered, 539 protein-coding sequences remained. Zebrafish orthologs of the most functionally interesting genes were selected for further analysis with Co-IPs.

    Journal: Scientific reports

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3.

    doi: 10.1038/s41598-023-27878-9

    Figure Lengend Snippet: Figure 1. Isolation of novel binding partners of HMX3 with yeast two-hybrid screening. Top: Schematic representation of bait and prey constructs used in screening. Full-length mouse HMX3 fused to the Gal4 DNA- binding domain (DBD) was used as bait. A library of stage E11 mouse cDNAs fused to the Gal4 activating domain (AD) was used as prey. 7.2 × 108 solid plate matings were performed, yielding 3200 positive colonies. Positive prey inserts were segregated and sequenced and reads were aligned to the mouse genome, identifying 851 unique genes. After removing non-coding genes and those for which only untranslated region (UTR) or intronic sequences were recovered, 539 protein-coding sequences remained. Zebrafish orthologs of the most functionally interesting genes were selected for further analysis with Co-IPs.

    Article Snippet: We used mouse E11 cDNA library (630478; Takara Bio) cloned into pGAD57 vector as prey, transformed into Y187 yeast strain.

    Techniques: Isolation, Binding Assay, Two Hybrid Screening, Construct